Fig. 1. PTEN expression is downregulated in AIA FLSs. (A) Representative H&E staining of AIA and normal synovial tissues in rat (original magnification, 20×). (B) PTEN expression in AIA and normal synovial tissues was analyzed by immunohistochemical staining in rats (original magnification, 20×). (C) PTEN and vimentin expression was analyzed by double immunofluorescence staining in rat AIA and normal FLSs (original magnification, 40×). (D) PTEN protein levels were analyzed by western blot in AIA and normal FLSs. (E) PTEN mRNA levels were analyzed by q-PCR in AIA and normal FLSs. All values are expressed as the mean ± SD. ^## P<0.01 vs. normal group.